ELISA-Entwicklung
GmbH & Co. KG
Range of Services
Assay Development
Assay Validation
Sample Measurement
Protein Purification
Antibody Production
HCP-Assays
Anti Drug Antibody, ADA
Testkits
This validation describes the general assay criteria which are determined routinely: sensitivity, measuring range, parallelism, recovery and intra assay variation coefficient. Cross reactions to similar substances are determined only if they occur. A reliable value for the interassay variation coefficient can only be obtained after completion of the assay development by carrying through the test with a large number of samples.
Antibody characterization is possible by electrophoresis and western blot.
Tips for carrying out enzyme-immune-assays
This validation means the experimental proof of known biological reactions by an already developed assay. For example in a stimulation experiment in vivo on animals or in cell culture media. We can barely carry through those examinations, because we neither practice animal husbandry nor cell culture.
Biological assay validation and characterization is possible at any time with samples made available by the customer.